Troubleshooting in bioprocessing
Sponsored by: Eppendorf AG
Date: 23 June
Days to go: 10
Time: 2PM London / 3PM CET
From pre-culture to production
Bioprocessing is an important tool in industrial biotechnology, from the production of therapeutic proteins and vaccines in the pharmaceutical industry to food and feed engineering, up to the sustainable production of fuels, chemicals, and polymers. Each application has its individual challenges and requirements, that must be considered for that specific process. All aim on the generation of the product in consistently high quality, sufficient yields, and in a fast, cost-efficient manner. Successful bioprocessing is complex and depends on many factors. From the selection of the expression host, media composition, the proper cultivation system and cultivation strategy, adequate downstream processes, up to the development of a robust tech transfer.
In this webinar we will address general and day-to-day challenges in the bioprocessing workflow from shake flask to production and discuss possible solutions. We will initially look on common challenges in reactor set-ups and configurations. What vessel and impeller type is the appropriate one for different culture demands (e.g. shear sensitivity, high oxygen demands), what process parameters control is essential and how the integration of additional analytical tools can help to improve process stability. We will address frequent challenges in cultivation, e.g. how to produce reproducible inoculums in shake flasks, how to prevent contamination in a bioreactor, during transfer or sampling and how to cope with difficulties in cultivation in the bioreactor, e.g. foaming or viscous solutions. We will further have a look on the different steps of a process and discuss optimization factors and measures, how to improve yields for example by measuring the kLa or choosing the right process strategy. Once a process is established, the aim is to transfer the process step-wise to larger volumes without losing efficiency and product quality. Successful scale-up poses several challenges, including the proper tech transfer of the operating conditions. Which scale-up strategy helps to overcome these challenges best, and what digital tools are available to ease scale transition? Finally, another critical bioprocessing bottleneck is the harvesting process. We will have a short glance on harvesting optimization in the end and evaluate, how to handle best high volumes by keeping downstream productivity.
So register now, if you want to have answers to frequently asked questions in bioprocessing and understand how to cope with challenges in bioprocessing from shake flask to bioreactor.
If you want to get answers on specific questions during the webinar, send an email to email@example.com by June 6.
Application Specialist Cell Handling, Eppendorf AG
Ines Hartmann is application specialist cell handling responsible for the shakers and mixers at the Eppendorf headquarter in Hamburg, Germany. She joined the company in 2008 and has worked on several topics in cell handling, liquid handling and consumables. She obtained her diploma degree in biology at the Humboldt University of Berlin, Germany and gained expertise in various laboratory techniques.
Bioprocess Field Application Specialist, Eppendorf North America
Ankita Desai is a Bioprocess Field Application Specialist at Eppendorf North America. She supports customers with her expertise in cell culture bioprocessing and applications. After her Master’s in Pharmaceutical Sciences at the Northeastern University in Boston, she has gained several experiences working for global players in the pharmaceutical industry.
Key Learning Objectives
- Get answers to frequently asked questions in bioprocessing.
- Learn how to define and optimize important parameters to improve culture results.
- Get hands-on advice to optimize each step of your process.
- Live “Ask the Expert” session – Deepen your knowledge and optimize your process.
- Heads of Research and Development
- Research Assistant
- Project Manager
- Head of Bioprocess Applications
- Head of Applications
- Service engineer
- Biotechnology students
- Laboratory Manager
- Laboratory Assistant