A Versatile Toolbox for Intensified CHO Process Development

Sponsored by: Sartorius Stedim Biotech

Focused on:

  • Biotherapeutics

Date: 9 July


Time: 3PM London/10AM New York

Selecting the right clones for unleashing productivity gains

Intensified process formats promise improved cost efficiency in biotherapeutics production by lowering cost of goods and increasing plant flexibility. CHO clones from traditional cell line development differ in responsiveness to process intensification and sometimes fail altogether. Therefore, tailored and time-efficient approaches are key for selecting suitable clones and developing associated USP processes.

This webinar introduces a versatile toolbox which addresses these requirements: ambr 15-based perfusion mimics enable to assess the suitability of clones for process intensification under near-process conditions. This setup also supports developing different types of intensified USP processes and optimizing media compositions. We demonstrate upscale from ambr 15 to a rocking motion-based reactor for perfusion applications. Furthermore, the webinar will illustrate how to apply the ambr 250 to developing a high-inoculation fed batch process including transfer to the 5L scale.

Using this toolbox, significant productivity improvements were achieved in intensified formats by repurposing CHO clones from fed batch selection: up to 3 fold in high inoculation fed batch and >4-fold in perfusion without optimization. Importantly, this ambr 15-based approach also enables spotting clones with limited performance gains or risk of low productivity in intensified setups already at the cell line development stage. This ensures you can move ahead with the right clones for your intensification project.
If you are interested in learning more about a quick route to unleash unutilized performance reserves of your CHO production clones, please sign up!

Presented by

Dirk Müller,

Manager Product Development Team 2, CTS, Sartorius Stedim Biotech

Dirk Müller heads a product development team at the Cell line and Testing Solutions unit of Sartorius Stedim Biotech. His team develops media and different process formats for CHO cells. Before joining Sartorius, he has worked in several positions at Insilico Biotechnology, a company specializing in optimization of bioprocesses using predictive cell models. Dirk holds a Master’s in chemical and process engineering and a PhD in biochemical engineering.

Key Learning Objectives

  • Learn how the ambr 15 can be applied to pick the right clones for intensified processes
  • See how intensified processes developed at ambr scale can be scaled up to various intensified process variants easily


  • Process Development Scientists
  • Process Development Managers
  • Process Engineers
  • Engineering Directors
  • Cell Line Development Scientist
  • Cell Line Development Manager